diff --git a/Lib/test/test_unittest/testmock/testhelpers.py b/Lib/test/test_unittest/testmock/testhelpers.py
index 0e82c723ec3eaa..f97161a9fee945 100644
--- a/Lib/test/test_unittest/testmock/testhelpers.py
+++ b/Lib/test/test_unittest/testmock/testhelpers.py
@@ -1,3 +1,4 @@
+import functools
 import inspect
 import time
 import types
@@ -954,6 +955,45 @@ def __getattr__(self, attribute):
         self.assertNotHasAttr(autospec, '__name__')
 
 
+    def test_autospec_partial_function_signature_enforced(self):
+        # A mock created from a functools.partial object enforces the
+        # partial's effective, post-partial-application signature: "method"
+        # is already bound to "POST", so only "url" and "payload" remain.
+        def send_request(method, url, payload=None):
+            return method, url, payload
+
+        send_post_request = partial(send_request, "POST")
+        send_post_request("https://example.com")
+        send_post_request("https://example.com", payload="data")
+
+        mocked = create_autospec(send_post_request)
+        mocked("https://example.com")
+        mocked("https://example.com", payload="data")
+        self.assertRaises(TypeError, mocked)
+        self.assertRaises(TypeError, mocked, "a", "b", "c")
+        self.assertRaises(TypeError, mocked, "https://example.com", foo="lish")
+
+
+    def test_autospec_partialmethod_self_skipped(self):
+        # _must_skip() didn't recognize functools.partialmethod class
+        # attributes, so `self` was never dropped from the enforced
+        # signature when autospeccing a whole class.
+        class Foo:
+            def method(self, a, b, c=3):
+                return a, b, c
+            partial_method = functools.partialmethod(method, 1)
+
+        real = Foo()
+        real.partial_method(2, 3)
+
+        mocked = create_autospec(Foo)
+        instance = mocked()
+        instance.partial_method(2)
+        instance.partial_method(2, c=4)
+        self.assertRaises(TypeError, instance.partial_method)
+        self.assertRaises(TypeError, instance.partial_method, 2, 3, 4)
+
+
     def test_autospec_signature_staticmethod(self):
         class Foo:
             @staticmethod
diff --git a/Lib/unittest/mock.py b/Lib/unittest/mock.py
index 49011faaa51eae..1a41189e060225 100644
--- a/Lib/unittest/mock.py
+++ b/Lib/unittest/mock.py
@@ -37,7 +37,7 @@
 from dataclasses import fields, is_dataclass
 from types import CodeType, ModuleType, MethodType
 from unittest.util import safe_repr
-from functools import wraps, partial
+from functools import partialmethod, wraps, partial
 from threading import RLock
 
 
@@ -107,6 +107,14 @@ def _get_signature_object(func, as_instance, eat_self):
             eat_self = True
         # Use the original decorated method to extract the correct function signature
         func = func.__func__
+    elif isinstance(func, partial):
+        # inspect.signature() already knows how to compute the effective,
+        # post-partial-application signature of a functools.partial object
+        # directly. Don't fall through to the generic func.__call__ lookup
+        # below: partial.__call__ is a C slot wrapper whose own signature is
+        # just the unenforceable "(*args, **kwargs)" of the functools.partial
+        # constructor, not of the wrapped callable.
+        pass
     elif not isinstance(func, FunctionTypes):
         # If we really want to model an instance of the passed type,
         # __call__ should be looked up, not __init__.
@@ -2917,9 +2925,12 @@ def _must_skip(spec, entry, is_type):
             continue
         if isinstance(result, (staticmethod, classmethod)):
             return False
-        elif isinstance(result, FunctionTypes):
+        elif isinstance(result, (FunctionTypes, partialmethod)):
             # Normal method => skip if looked up on type
             # (if looked up on instance, self is already skipped)
+            # functools.partialmethod resolves to a plain function carrying
+            # `self` when looked up via getattr() on the type, see
+            # functools.partialmethod.__get__.
             return is_type
         else:
             return False
diff --git a/Misc/NEWS.d/next/Library/2026-07-10-11-12-13.gh-issue-79644.mWr00f.rst b/Misc/NEWS.d/next/Library/2026-07-10-11-12-13.gh-issue-79644.mWr00f.rst
new file mode 100644
index 00000000000000..26122f0e2d9ef7
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2026-07-10-11-12-13.gh-issue-79644.mWr00f.rst
@@ -0,0 +1,9 @@
+Fix :func:`unittest.mock.create_autospec` (and ``mock.patch(...,
+autospec=True)``) computing the wrong signature for :class:`functools.partial`
+and :class:`functools.partialmethod` targets. A ``functools.partial`` mock
+previously accepted calls with any number of arguments, since its
+effective, post-partial-application signature was never consulted. A
+``functools.partialmethod`` class attribute, when autospecced as part of a
+whole class, previously had its ``self`` parameter checked against the
+partialmethod's own pre-bound argument instead of being skipped, so calls
+were validated against the wrong signature shape.